General description
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1 receptor protein.
Recognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
This Anti-Dopamine D₁ Receptor Antibody is validated for use in ELISA, WB, Frozen Sections, Free-Floating Sections, Paraffin Sections for the detection of Dopamine D₁ Receptor.
Immunogen
Human
a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
Application
ELISA (see comments)
Immunoblotting (1:1000)
Frozen Sections (1:5000)
Free-Floating Sections (1:5000)
Paraffin Sections (not recommended)
Physical form
Undiluted serum.
Preparation Note
Reconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Analysis Note
Positive Control
Rat brain
Other Notes
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | lyophilized |
| does not contain | preservative |
| species reactivity | human, mouse, rat |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| dilution | (ELISA Immunoblotting (1:1000) Frozen Sections (1:5000) Free-Floating Sections (1:5000) Paraffin Sections (not recommended)) |
| isotype | IgG |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... DRD1(1812) rat ... Drd1(24316) |
