General description
Adenosylhomocysteinase (UniProt: P23526; also known as EC: 3.3.1.1, AdoHcyase, S-adenosyl-L-homocysteine hydrolase) is encoded by the AHCY (also known as SAHH) gene (Gene ID: 191) in human. Adenosylhomocysteinase is a homotetrametic enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine. Hence, it regulates the intracellular S-adenosylhomocysteine concentration, which is thought to be important for transmethylation reactions. Two isoforms of adenosylhomocysteinase have been reported that are produced by alternative splicing. It contains three nucleotide binding regions (aa 157-159; 222-227; and 299-301). Defects in AHCY gene cause hypermethioninemia that is characterized with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
~47 kDa observed; 47.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminus region of human Adenosylhomocysteinase.
Application
Anti-Adenosylhomocysteinase (AHCY) Antibody, Cat. No. ABE1803, is a highly specific rabbit polyclonal antibody that targets Adenosylhomocysteinase (AHCY) and has been tested for use in Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 2 µg/mL from a representative lot detected Adenosylhomocysteinase (AHCY) in 200 µg of human placental tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Adenosylhomocysteinase (AHCY) in human kidney and human skin tissues.
Biochem/physiol Actions
This rabbit polyclonal antibody detects Adenosylhomocysteinase in human. It targets and epitope within 12 amino acids from the C-terminus region.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Adenosylhomocysteinase (AHCY) in 200 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | chimpanzee (based on 100% sequence homology) |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_000678.1 |
| UniProt accession no. | P23526 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... AHCY(191) |
