General description
MAFA is a tissue specific transcription factor found in the nuclei of pancreas islet beta cells. MAFA specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. MAFA also cooperates synergistically with NEUROD1 and PDX1 in enhancing additional gene expression during development as well. Phosphorylation by GSK3 increases MAFA transcriptional activity and is required for its oncogenic activity. MAFA can be either an oncogene or act as a tumor suppressor, depending on the cell context.
~ 35 kDa observed
Immunogen
Epitope: Region near the N-terminus of MAFA
Mixture of three linear peptides corresponding to amino acids 1-150 of human MAFA.
Application
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot was reported to detect MAFA in HeLa, 293T/17, Capan-2, SAOS-2, SH-SY5Y, and Skin 3, 44 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
This Anti-MAFA Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of MAFA.
Biochem/physiol Actions
Recognizes MAFA
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in a HEK293T cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected MAFA in a 293T cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_963883 |
| UniProt accession no. | Q8NHW3 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MAFA(389692) |
