Application
Anti-Human IgG (Fc specific)-Cy3 antibody produced in goat was used to screen sera on glycopeptide microarrays at a dilution of 1:1000. It was used at a working dilution of 1:100 for immunohistochemistry analysis of mouse footpad sections incubated with human polyclonal anti-Leishmania serum.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Human IgG (Fc specific) antibody allows for the visualization of proteins by fluorescent microscopy.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Legal Information
Cy is distributed under license from Amersham Biosciences Limited.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Level | 200 |
| conjugate | CY3 conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
