Biochem/physiol Actions
Phosphorylates a spectrum of substrates, and regulates many cellular processes.
Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.
Physical form
Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.
Analysis Note
One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.
| recombinant | expressed in E. coli |
| Quality Segment | 200 |
| assay | >90% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| specific activity | >1000 units/mg protein |
| mol wt | 43.5 kDa |
| UniProt accession no. | P17612 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PRKACA(5566) |
