General description
Human Jak2 (GenBank Accession No. NM_004972), containing the catalytic and the pseudo kinase domain (amino acids 532-1132) with N-terminal GST-His tag, MW = 99 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.
| biological source | human |
| recombinant | expressed in baculovirus infected insect cells |
| assay | ≥70% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 99 kDa |
| packaging | pkg of 10 μg |
| concentration | >0.02 mg/mL |
| NCBI accession no. | NM_004972 |
| UniProt accession no. | O60674 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... JAK2(3717) |
