General description
CD19 is transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Immunogen
Human CCL (chronic lymphocytic leukemia) cells
Application
The reagent is designed for Flow Cytometry analysis. working concentrations should be determined by the investigator.
Biochem/physiol Actions
The antibody 4G7 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
Features and Benefits
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Physical form
Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | biotin conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 4G7, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| concentration | 1 mg/mL |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_001178098.1 |
| UniProt accession no. | P15391 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CD19(930) |
