General description
Lymphotoxin-a (UniProt: P01374; also known as LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1) is encoded by the LTA (also known as TNFB, TNFSF1) gene (Gene ID: 4049) in human. Lymphotoxin-a is cytokine of the tumor necrosis family that is synthesized by lymphocytes with a signal peptide (aa 1-34), which is subsequently cleaved off to generate the mature form. It is shown to be cytotoxic for a wide range of tumor cells in vitro and in vivo. It can exist as a secreted homotrimeric form or can form a heterotrimer with lymphotoxin-a to become a membrane associated form. The homotrimeric form is shown to bind to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM and the heterotrimer binds exclusively to TNFRSF3/Lymphotoxin- receptor (LTBR). Lymphotoxin- is reported to be involved in the formation of secondary lymphoid organs during development and play a role in apoptosis. Mutations in LTA gene are known to cause Psoriatic arthritis, an inflammatory seronegative arthritis, which is a heterogeneous disorder that can range from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human TNFβ (Lymphotoxin-a).
Application
Quality Control Testing
Evaluated by Western Blotting with human recombinant TNFβ/LTA.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human TNFβ/LTA.
Tested applications
Flow Cytometry Analysis: 1 mg from a representative lot detected TNFβ/LTA in one million HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TNFβ/LTA in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TNFβ/LTA, clone 3B20-H2 ZooMAb®, Cat. No. ZRB1195, is Rabbit monoclonal antibody that specifically targets TNFβ/LTA and is tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Biochem/physiol Actions
Clone 3B20-H2 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects TNFβ/LTA.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3B20-H2, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 22.30 kDa,observed mol wt ~17 kDa |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | flow cytometry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_000586.2 |
| UniProt accession no. | P01374 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
