General description
Homeobox protein SIX1 (Uniprot: Q15475; also known as Sine oculis homeobox homolog 1, SIX1) is a transcription factor that is encoded by the gene SIX1 (Gene ID: 6495) in human. SIX1 belongs to a family of evolutionarily conserved transcription factors that are found in lower and higher organisms. A total of six family members (SIX1-6) have been identified in vertebrates that contain a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions. It can function both as a transcriptional repressor or activator depending on the context. This protein plays a crucial role in the regulation of cell proliferation, apoptosis, and embryonic development. It acts by regulating the expression of target genes involved in organogenesis and morphogenesis. SIX1 is primarily known for its role in the development of multiple organs, such as the kidney, ear, and skeletal muscles. SIX1 mediates apoptosis, autophagy, and regulates resistance to the drug in hepatocellular carcinoma. It is highly expressed in colorectal cancer and promotes tumor progression. Abnormal expression of SIX1 is also associated with other cancer subtypes, including Hodgkin lymphoma, breast, and ovarian cancer. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal half of human Homeobox protein SIX1.
Application
Quality Control Testing
Evaluated by Western Blotting in L6 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Homeobox protein SIX1 (SIX1) in L6 cell lysate.
Tested Applications
Affinity Binding Assay Analysis: The binding affinity of this antibody to SIX1 peptide was at least ten thousand-fold (10000X) higher than that obtained with the non-specific control peptide.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SIX1 in human cervix tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SIX1 in C2C12 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1M23 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects Homeobox protein SIX1 (SIX1). It targets an epitope within 25 amino acids from the C-terminal half of the protein.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1M23, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 32 kDa,observed mol wt ~37 kDa |
| purified by | using Protein A |
| species reactivity | human, rat, mouse |
| species reactivity (predicted by homology) | porcine, sheep, canine |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_005973 |
| UniProt accession no. | Q15475 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... SIX1(6495) |
