General description
Research area: CELL SIGNALING
Human cathepsin F (GenBank Accession No. NM_003793), CD33 signal peptide(amino acids 1-16) + cathepsin S (amino acids 20-end), with C-terminal HIS tag, MW = 53 kDa (more than 58 kDa after post-modification), expressed in FreeStyle 293-F cells.
Application
Active human cathepsin F is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin F has been used in a study to assess its implications for the development of novel immunomodulators. Active human cathepsin F has also been used in a study to investigate microarray expression analysis and identification of serum biomarkers for Niemann-Pick disease.
Biochem/physiol Actions
Cathepsin F (CtsF) are endopeptidases that play a role in lysosomal protein degradation. Beyond its involvement in protein recycling, CtsF has also been associated with immune responses mediated by Toll-like receptors (TLRs) and antigen processing. Additionally, mutation in the cathepsin F gene is related to the development of familial papillary thyroid cancer , type B Kufs disease, defective lysosomal degradation and abnormal autophagy, and Alzheimer′s disease.
Physical form
Formulated in 45 mM sodium acetate, 135 mM NaCl and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C
Legal Information
FreeStyle is a trademark of Invitrogen Corp.
| recombinant | expressed in FreeStyle™ 293-F cells |
| assay | ≥90% (SDS-PAGE) |
| form | aqueous solution |
| specific activity | ≥18 pmol/min-μg |
| mol wt | 53 kDa |
| technique(s) | activity assay: suitable |
| solubility | soluble,water: soluble |
| NCBI accession no. | NM_003793 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... CTSF(8722) |
