Application
Useful for kinase assay.
Biochem/physiol Actions
Recombinant human EGFR (C797S) (668-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | 70-95% (SDS-PAGE) |
| form | frozen liquid |
| specific activity | 74-102 nmol/min·mg (radiometric) |
| mol wt | ~89-100 kDa |
| concentration | 0.1 μg/μL |
| accession no. | NM_005228 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... EGFR(1956) |
