General description
The antibody MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types.
The previously assigned protein identifier P01891 has been merged into P04439. Full details can be found on the UniProt database.
Immunogen
PHA-activated peripheral blood lymphocytes
Application
The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Features and Benefits
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Physical form
Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | phycoerythrin (R-PE) conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | MEM-147, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| concentration | 0.1 mg/mL |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_002116.6,NM_002117.4,NM_005514.6 |
| UniProt accession no. | P04439 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... HLA-A(3105), HLA-B(3106), HLA-C(3107) |
