General description
MANF has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease.
Biochem/physiol Actions
MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. Recombinant human MANF is an 18.1 kDa protein consisting of 158 amino acids including 8 cysteine residues.
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 7.0.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC),≥98% (SDS-PAGE) |
| form | lyophilized |
| potency | 15-25 μg/mL ED50 |
| mol wt | 18.1 kDa |
| packaging | pkg of 25 μg |
| impurities | <0.1 EU/μg endotoxin, tested |
| color | white to off-white |
| UniProt accession no. | P55145 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... MANF(7873) |
