General description
Clustered, regularly interspaced, short palindromic repeat (CRISPR) systems, encode RNA-guided endonucleases that are essential for bacterial adaptive immunity. Ddepending on the architecture of the effector-CRISPR RNA (crRNA) interference module, the ddifferent CRISPR-Cas systems could be assigned into two classes: Class-1 systems of multi-subunit complex such as Cascade and Class-2 systems of single enzyme, such as Cas9.
Cpf1 (CRISPR from Prevotella and Francisella 1) belongs to Class-2 type V CRISPR-Cas endonuclease system.4-5 Cpf1 comprise several differences from Cas9 protein including cleavage with 5′overhangs, a shorter guide RNA and a longer distance between the seed sequence and the cleavage site.
AsCpf1, Cpf1 from Acidaminococcus sp. (strain BV3L6), was examined together with 15 members of Cpf1 nuclease family and shown to mediate efficient genome editing in HEK293FT cells with an improved results compared to SpCas9.5 AsCpf1 crystal structure in a complex with crRNA and partially duplexed target DNA, shows that AsCpf1 acts as a monomer thus identifying a unique mechanism employed by AsCpf1 for target recognition.
Immunogen
recombinant Cpf1 from Acidaminococcus sp. (strain BV3L6)
Application
Monoclonal Anti-AsCpf1 recognizes Cpf1 from Acidaminococcus sp. (strain BV3L6). The product has been used in several immunochemical techniques including
- immunoblotting (~135 kDa),
- immunofluorescence
- immunoprecipitation
Monoclonal anti-AsCpf1 antibody can provide a useful tool for genome editing research such as detecting and monitoring AsCpf1 positively transfected cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | AsCpf-11, monoclonal |
| form | buffered aqueous solution |
| mol wt | ~135 kDa |
| concentration | ~1.0 mg/mL |
| technique(s) | immunoblotting: 1.25-2.5 μg/mL using purified recombinant AsCpf1 produced in E. coli,immunofluorescence: 1.25-2.5 μg/mL using human HEK-293T cells over-expressing AsCpf1 protein,immunoprecipitation (IP): 2.5-5 μg/test using lysate of human HEK-293T cells over-expressing AsCpf1 protein |
| isotype | IgG2a |
| UniProt accession no. | U2UMQ6 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
