General description
CAPN6 Antibody: Calpains make up a ubiquitously expressed, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. This large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes as their activation can be triggered by calcium influx and oxidative stress. Calpain 6 (CAPN6) is most similar to Calpain 5; the C-terminal region of CAPN6 lacks homology to the calmodulin-like domain of other vertebrate calpains. CAPN6 is thought to be involved in the regulation of microtubule dynamics and cytoskeletal organization. CAPN6 has also been recently identified as an HIV dependency factor (HDF), suggesting that CAPN6 may be an important drug target in HIV treatment.
Immunogen
CAPN6 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human CAPN6.The immunogen is located within the last 50 amino acids of CAPN6.
Application
CAPN6 antibody can be used for the detection of StrepII by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Physical form
Supplied in PBS containing 0.02% sodium azide.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| species reactivity | mouse, human, rat |
| concentration | 1 mg/mL |
| technique(s) | immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_055104 |
| UniProt accession no. | Q9Y6Q1 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CAPN6(827) |
