General description
Calreticulin (CALR) is expressed in the endoplasmic reticulum. The gene encoding it is localized on human chromosome 19p13.13.
Application
Human calreticulin has been used in a study to determine the effects of uncoupling protein 4 (UCP4) on the expression of proteins in the store-operated Ca2+ entry pathway.
Biochem/physiol Actions
Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the ′calreticulin/calnexin cycle′ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis.
Physical form
Solution in 55 mM TRIS-HCl, pH 8.2, containing 150 mM NaCl.
Other Notes
Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag.
| biological source | human |
| recombinant | expressed in E. coli |
| tag | His tagged |
| assay | >90% (SDS-PAGE) |
| form | liquid |
| mol wt | ~55 kDa by SDS-PAGE |
| packaging | pkg of 10 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| technique(s) | calcium flux assay: suitable |
| impurities | <1 EU/μg endotoxin, tested |
| UniProt accession no. | P27797 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... CALR(811) |
