General description
Intracellular Calcium (Ca2+) acts as major secondary messenger that mediates signalling induced by hormones and neurotransmitters. Cellular responses to calcium are modulated by a family of Ca2+/Calmodulin dependent protein kinases. The members of this family include CaMK I, CAMK II and CAMK IV. These kinases mediate rapid, highly specific and reversible post-translational modification involving ATP. CaMK II is abundantly expressed in mammalian brain, with highest expression in neurons of hippocampus and cerebral cortex, and plays critical role in synaptic transmission, memory, learning and behaviour. CaMK II family has four members, CaMK IIα, CaMK IIβ, CaMK IIγ and CaMK IIδ. The CaMK IIα?and β?isoforms are predominantly expressed in the brain, localized mainly in the cytosol and postsynaptic densities (PSDs), whereas the CaMK IIγ and δ isoforms are expressed in all tissues. CaMK II is autophosphorylated at α-Thr286 residue in response to changes in the concentration of intracellular calcium. This may prolong physiological responses to temporary changes in Ca2+ concentrations. CaMK II regulates synaptic signalling by phosphorylating glutamate receptors in the neurons. A distinct CaMK II has also been implicated in the process of smooth muscle contraction by phosphorylation of myosin light chain kinase.
This product reacts with the recombinant and native rat phosphorylated CaMK II α subunit only (50 kDa). It does not cross-react with the non-phosphorylated α subunit or with the 60 kDa β subunit in either phosphorylation state.
Immunogen
phosphorylated (at Thr286) synthetic phosphopeptide corresponding to amino acids of rat and mouse CaM kinase IIα subunit that contains threonine 286.
Application
Anti-phospho-CaM Kinase IIα (pThr286) antibody may be used in indirect immunofluorescence at a working dilution of 1:100 in rat hippocampal cells fixed with cold methanol. Detection by immunoblotting is possible at a working dilution of 1:1000.
Biochem/physiol Actions
The antibody reacts specifically with both the recombinant and native rat phosphorylated CaM (Ca2+/calmodulin) dependent kinase IIα subunit. It does not cross-react with the non-phosphorylated α subunit or with the β subunit (60 kDa) in either phosphorylation state. By immunofluorescence on rat hippocampal cells, the antibody labels cell somas and neuropil areas, while nuclei are only lightly stained.
Physical form
Solution in phosphate buffered saline containing 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 22B1, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| species reactivity | rat, mouse |
| technique(s) | indirect immunofluorescence: 1:100 using rat hippocampus (cold methanol fixation),western blot: 1:2,000 |
| isotype | IgG1 |
| UniProt accession no. | P11798 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr286) |
| Gene Information | mouse ... Camk2a(12322) rat ... Camk2a(25400) |
