General description
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Immunogen
recombinant human cyclin D1 (amino acids 1-295)
Biochem/physiol Actions
The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Physical form
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | phycoerythrin (R-PE) conjugate |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | DCS-6, monoclonal |
| form | buffered aqueous solution |
| species reactivity | ( Human), ( Mouse), rat, ( Non-human primates) |
| concentration | 0.1 mg/ml |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2a |
| UniProt accession no. | P24385 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CCND1(595) |
