Application
Folate binding protein from bovine milk has been used in folate binding studies using surface plasmon resonance and in the uptake studies in kidney proximal tubule cells.
Folate binding protein can be used in radioligand binding assays of serum folate. The folate receptor has been used in studies as a therapeutic target in a variety of cancers.
Useful for radioligand binding assays of serum folate. May also bind N-methyltetrahydrofolic acid and other folate analogues.
Biochem/physiol Actions
Folate-binding protein (FBP) is present as a cell receptor and binds to folate in equimolar ratio. It is involved in the transport of folate and improves their bioavailability.
Packaging
Package size based on protein content
Physical form
Lyophilized powder containing buffer salt as sodium citrate
Analysis Note
Purified over 1,000 fold. May contain a trace of β-mercaptoethanol.
| biological source | bovine milk |
| form | lyophilized powder |
| usage | mg sufficient for ~4,000 assays |
| composition | Protein, 20-40% modified Warburg-Christian |
| technique(s) | ligand binding assay: suitable,surface plasmon resonance (SPR): suitable |
| capacity | 1.5-6.0 μg/mg, protein binding capacity (folic acid)(25 °C at pH 7.5.) |
| storage temp. | −20°C |
