General description
Transferrin receptor (TFRC, p90) is also known as cluster of differentiation 71 (CD71). The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. In human chromosome, the gene TFRC is localized on 3q29.
Immunogen
NALM-6 human pre-B cell line
Application
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.
Biochem/physiol Actions
Transferrin receptor (TFRC, CD71) facilitates the uptake of iron bound to transferrin (Tf) through clathrin-mediated endocytosis. TFRC interacts with iron-bound holo-transferrin at pH 7.4 and does not interact apo-transferrin. TFRC also binds with H-ferritin. Expression level of TFRC is regulated stringently by intracellular iron levels. The expression is higher in erythroblasts owing to haemoglobin synthesis. The expression is also higher in cancer cells, osteoclasts and activated lymphocytes, where iron requirement is high. Hypoxia and iron deficiency also increases TFRC expression. Recycling of Tf and TFRC is essential for the uptake of iron from the serum. Mutations in the internalization motif of TFRC leads to impaired endocytosis and consequently congenital/combined immunodeficiency. Improper palmitoylation of TFRC is connected to neurodegeneration with brain iron accumulation. Upregulation of TFRC is implicated in progression of pancreatic cancer, adult T-cell leukemia/lymphoma (ATLL), and cholangiocarcinoma.
Features and Benefits
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Physical form
Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | FITC conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | MEM-75, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_001128148.1 |
| UniProt accession no. | P02786 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TFRC(7037) |
