General description
The gene encoding Retinoblastoma-associated protein (RB1) is a tumor suppressor gene localized on human chromosome 13q14.2. Human recombinant RB protein (773-end) with N-terminal GST tag (GenBank Accession No. NM_000321), expressed in a E. coli expression system.
Application
Retinoblastoma-associated protein (RB1) has been used in cell activity assay. It is also used as a kinase substrate for the kinase assay and in Western blotting.
Biochem/physiol Actions
Retinoblastoma-associated protein (RB1) modulates the cell cycle. It acts as a repressor of transcription mediated by E2 factor (E2F) and influences transcriptional silencing. RB1 modulates mitochondrial activities and has a role in the organisation of heterochromatin. Mutations in the gene encoding this protein have been associated with childhood retinoblastoma.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥80% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 43.5 kDa |
| packaging | pkg of 200 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | >0.02 mg/mL |
| NCBI accession no. | NM_000321 |
| UniProt accession no. | P06400 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... RB1(5925) |
