General description
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
Immunogen
A phospho specific peptide corresponding to residues surrounding S259 of human Phospho-Raf1-S259
Physical form
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | monoclonal |
| form | liquid |
| species reactivity | human, mouse |
| concentration | 1.25 mg/mL |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P04049 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer259) |
| Gene Information | human ... RAF1(5894) |
