产品介绍
Product Description
Biochem/physiol Actions
AMPK (A1/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and 3-hydroxy-3-methylglutaryl-CoA reductase. The β subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.
Physical form
Supplied in 50 mM Sodium Phosphate, pH 7.0, with 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
技术参数
Specifications
| recombinant | expressed in baculovirus infected Sf9 cells |
| Quality Segment | 200 |
| product line | PRECISIO® Kinase |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| specific activity | 297-401 nmol/min·mg |
| mol wt | A1 subunit ~68 kDa,B2 subunit ~36 kDa,G1 subunit ~40 kDa |
| UniProt accession no. | O43741,P54619,Q13131 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PRKAA1(5562), PRKAB2(5565), PRKAG1(5571) |
