General description
Human Plk1 polo box domain (GenBank Accession No. NM_005030), amino acids 367-end with N-terminal GST-tag, MW=53 kDa, expressed in an E. coli expression system.
Polo-like kinase 1 (PLK1) belongs to the PLK family. It comprises polo-box domain (PBD). PBD, in turn, contains polo boxes (PB1 and PB2) with a 6-stranded β sandwich and an α helix as structural elements. The PLK1 gene is mapped to human chromosome 16p12.2.
Application
PLK1 Polo Box Domain active human has been used in the in vitro binding assay with various synthetic peptides coupled to streptavidin beads. It has also been used in binding studies with fluorescein-labeled substrate peptide 5-carboxyfluorescein-GPMQSpTPLNG to test the effect of various inhibitors.
Biochem/physiol Actions
Polo-like kinase 1 (PLK1) is a mitotic kinase that mediates the regulation of various events in mitosis including chromosome segregation, cytokinesis, centrosome maturation, and separation. It activates cell division cycle 25 (CDC25) phosphatase which further regulates mitotic entry. Elevated expression of PLK1 is observed in many tumors.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥90% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 53 kDa |
| packaging | pkg of 100 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | 2.9 mg/mL |
| NCBI accession no. | NM_005030 |
| UniProt accession no. | P53350 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PLK1(5347) |
