General description
Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol.
The molecular weight of mannitol dehydrogenase is 136 kDa.
Application
Can be used for the production of D-mannitol.
It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
This preparation is useful in the determination of mannitol in urine.
Biochem/physiol Actions
Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of
Physical form
含缓冲盐、磷酸钾以及二硫苏糖醇的冻干粉
Preparation Note
部分纯化
Analysis Note
根据 Yamanaka, Meth. Enzymol., 41(B), 138 (1975) 所述方法进行分析。
Other Notes
在 pH 5.3 和 30°C 且存在 NADH 的条件下，一单位本品每分钟将还原 1.0μmol
| form | lyophilized powder |
| Quality Segment | 200 |
| specific activity | ≥60 units/mg protein |
| mol wt | 136 kDa |
| storage temp. | −20°C |
