单克隆抗-HA，100UG，H7411-100UG，Sigma

General description

Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.
Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.

Immunogen

与人流感病毒血凝素(HA)片段相对应的合成肽，称为HA-标签，与KLH结合

Application

Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:

• in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells
• in western blotting detection of spindle-associated proteins in Trypanosoma brucei
• in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies

Biochem/physiol Actions

Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies.
该抗体可识别HA-标签蛋白的天然和还原变性形式，并且可与 大肠杆菌 或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.