General description
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5′-GGAG-3′ motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5′-GGAG-3′ motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Biochem/physiol Actions
This LIN28B antibody is generated from a mouse immunized with a recombinant protein of human LIN28B.
Physical form
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
| biological source | (Human) |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | monoclonal |
| form | liquid |
| mol wt | 27084Da |
| species reactivity | human |
| technique(s) | western blot: 1:500-1:2000 |
| isotype | IgG1, kappa |
| storage temp. | -10 to -25°C |
| target post-translational modification | unmodified |
| Gene Information | human ... LIN28B(389421) |
