General description
Serine/threonine-protein phosphatase PGAM5, mitochondrial (UniProt: Q96HS1; also known as EC:3.1.3.16, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5) is encoded by the pgam5 GENE (Gene ID: 192111) in human. PGAM5 is a single-pass mitochondrial membrane protein that displays phosphatase activity for serine/threonine residues and is shown to dephosphorylate and activate MAP3K5 kinase. It does not display any phosphoglycerate mutase activity. PGAM5 usually exists as a dimer, but can also form ternary complex with Nuclear Factor, Erythroid 2 Like 2 (NFE2L2) and Kelch Like ECH Associated Protein 1 (KEAP1). Its transmembrane domain is localized to amino acids 7 to 29. A 35 amino acid sequence at its N-terminal function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane. Two isoforms of PGAM5 have been described that are produced by alternative splicing. Both isoforms are reported to be phosphorylated by RIPK1/RIPK3 complex under conditions of necrosis and this phosphorylation is shown to increase PGAM5 phosphatase activity. Overexpression of isoform 1 results in clustering of mitochondria around the nucleus whereas isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
His-tagged recombinant fragment corresponding to 86 amino acids from the N-terminal half of human Phosphoglycerate mutase family member 5 (PGAM5).
Application
Anti-PGAM5, clone 2J4 ZooMAb, Cat. No. ZRB1055, is a Rabbit monoclonal antibody that specifically targets PGAM5 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PGAM5 in lysates from HEK293 cells, mouse testis, and human brain tissue.
Immunohistochemistry (Paraffin) Analysis: 1:100 dilution from a representative lot detected PGAM5 in human testis and human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound human recombinant PGAM5 with a KD of 3.0 x 10-9 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PGAM5 in MCF7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 2J4 is a ZooMAb rabbit recombinant monoclonal antibody that detects both isoforms of human Phosphoglycerate mutase family member 5 (PGAM5). It targets an epitope within 86 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2J4, recombinant monoclonal,monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 28.02 kDa,calculated mol wt 32 kDa,observed mol wt ~32 kDa,observed mol wt ~34 kDa |
| species reactivity | mouse, human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | Q96HS1 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PGAM5(192111) |
