General description
TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a highaffinity receptor for TNF alpha and TNF beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.
Immunogen
recombinant human TNF soluble receptor II (TNF sRII) expressed in Escherichia coli. The immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the transmembrane domain.
Application
Western Blot: 1 ug/mL detects rhs TNF RII at 5 ng/lane under non-reducing conditions
Immunocytochemistry: Concentration of 5-15 ug/ml detects TNF RII in human peripheral blood lymphocytes
Optimal dilutions should be determined by the end user.
Biochem/physiol Actions
The antibody detects human TNF sRII and shows no cross-reactivity with recombinant human TNF sRI.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) and 5% trehalose.
Other Notes
Protein A or G purified
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | lyophilized powder |
| species reactivity | human |
| technique(s) | immunocytochemistry: 5-15 μg/mL (detects TNF RII in human PBLs),western blot: 1 μg/mL (detects rhs TNF RII at 5 ng/lane under non-reducing conditions) |
| UniProt accession no. | P20333 |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TNFRSF1B(7133) |
