General description
Tyrosine-protein kinase transmembrane receptor ROR2 (UniProt: Q01974; also known as EC:2.7.10.1, Neurotrophic tyrosine kinase, receptor-related 2) is encoded by the ROR2 (also known as NTRKR2) gene (Gene ID: 4920) in human. ROR2 is a homodimeric, single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 34-403), a transmembrane domain (aa 404-424), and a cytoplasmic domain (aa 425-943). Its protein kinase domain is localized in amino acids 473-746. ROR2 is expressed at high levels during embryonic development and its expression levels decrease significantly around day 16 and adults display much reduced levels. ROR2 is involved in the early formation of chondrocytes and may be involved in cartilage and growth plate development. It phosphorylates tyrosine 3-Monooxygenase (YWHAB), leading to induction of osteogenesis and bone formation. It displays very little tyrosine kinase activity in vitro. Higher expression of ROR2 has been reported in several cancers, including solid malignancies, such as renal cell adenocarcinoma, breast cancer, and hematologic malignancies, such as multiple myeloma. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goydel, RS., et al. (2020). J. Biol. Chem. 295(18); 5995-6006; Peng, H., et al. (2017). J Mol. Biol. 429(19); 2954-2973; Liu, Y., et al. (2007). Mol. Endocrinol. 21(12); 3050-3061).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
From naïve rabbit antibody library (as described by Peng, H., et al. (2017). J. Mol. Biol. 429(19): 2954 2973).
Application
Quality Control Testing
Evaluated by Immunocytochemistry in T47D cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected ROR2 in T47D cells.
Tested Applications
Flow Cytometry Analysis: 1 µg from a representative lot detected ROR2 in one million T47D cells.
Western Blotting Analysis: A representative lot detected ROR2 in Western Blotting applications (Peng, H., et. al. (2017). J Mol Biol. 429 (19); 2954-2973).
Affinity Binding Assay: A representative lot of this antibody bound recombinant fragment of Human ROR2 with a KD of 2.5 x 10-6 in an affinity binding assay.
Flow Cytometry Analysis: A representative lot detected ROR2 in Flow Cytometry applications (Peng, H., et. al. (2017). J Mol Biol. 429(19); 2954-2973).
ELISA Analysis: A representative lot detected ROR2 in ELISA applications (Peng, H., et. al. (2017). J. Mol Biol. 429(19); 2954-2973).
Enzyme Immunoassay Analysis: Various dilutions of this antibody detected recombinant fragment of Human ROR2.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone XBR2-401 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Tyrosine-protein kinase transmembrane receptor ROR2. It targets an epitope within the Kringle domain from the extracellular region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | XBR2-401, recombinant monoclonal |
| description | XBR2-401 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 104.76 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable |
| isotype | IgG |
| epitope sequence | Extracellular domain |
| Protein ID accession no. | NP_004551.2 |
| UniProt accession no. | Q01974 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... ROR2(4920) |
