General description
Membrane cofactor protein (UniProt: P15529; also known as TLX, Trophoblast leukocyte common antigen, CD46) is encoded by the CD46 (also known as MCP, MIC10) gene (Gene ID: 4179) in human. CD46 is a single-pass type I membrane glycoprotein that acts as a cofactor for complement factor I, a serine protease that protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It is expressed in all nucleated cells and is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 35-343), a transmembrane domain (aa 344-366), and a cytoplasmic domain (aa 367-392). The cytoplasmic domain has two tails that are termed as Cyt1 and Cyt2. CD46 also contains four Sushi domains that are required for its interaction with various human viruses. Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein; Sushi domains 2 and 3 are essential for Herpesvirus 6 binding and domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are considered to be the most important for interaction with C3b and C4b. CD46 exhibits both signaling and receptor properties in immune and nonimmune cells, most of which are dependent on the expression of its cytoplasmic tails. Activation of CD46 is reported to promote T cell activation and differentiation and co-stimulation of TCR with CD46 can lead to T cell proliferation. Activated CD46 also leads to Tr1 Treg differentiation and secretion of IL-10 and granzyme B. The Tr1 differentiation initiated by CD46 is attributed to its Cyt1 domain. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Choileain, SN., et al. (2011). PLoS One 6(1); e16287; Weyand, NJ et al. (2010). J. Immunol. 184 (2); 694-701; Weyand, NJ., et al. (2006). Infect. Immun. 74(4);2428-2435).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the extracellular domain within the C-terminal half of human CD46.
Application
Quality Control Testing
Evaluated by Western Blotting in HL-60 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD46 in HL-60 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD46 in K562 cells.
Flow Cytometry Analysis: 1 µg from a representative lot detected CD46 in one million Human peripheral blood mononuclear cells (PBMC).
Affinity Binding Assay: A representative lot of this antibody bound CD46 peptide with a KD of 1.3 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD46 in human placenta tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1C10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects CD46. It targets an epitope within 24 amino acids from the extracellular domain within the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1C10, recombinant monoclonal |
| description | 1C10 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 43.75 kDa,observed mol wt ~55 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal extracellular domain |
| Protein ID accession no. | NP_002380 |
| UniProt accession no. | P15529 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CD46(4179) |
