Application
Human ZAP-70 Tyrosine Kinase was used for mapping the phosphorylation sites on LAT (linker for activation of T cells) for investigating the mechanism of activation of signalling proteins in T cells.
Biochem/physiol Actions
ZAP-70 Tyrosine Kinase is a protein belongs to Syk family and is essential for T cell activation. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. Additionally, it also plays a pivotal role in LFA-1-dependent T Cell Migration.
ZAP-70 is protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells. It is required for T cell activation. It is activated by Lck-mediated phosphorylation of its tyrosine residues.
Physical form
Solution in 25 mM HEPES, pH 7.5, containing 150 mM NaCl, 0.5 mM DTT, and 50% glycerol.
Other Notes
One unit will phosphorylate 1 nmol polyglutamic acid:tyrosine per min at 37 °C at pH 7.0.
| recombinant | expressed in baculovirus infected insect cells (Histidine tagged) |
| Quality Segment | 200 |
| assay | ≥90% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| specific activity | ≥150 U/mg |
| mol wt | 70 kDa |
| UniProt accession no. | P43403 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... ZAP70(7535) |
