Application
Study enzyme kinetics, and screen small molecular inhibitors of Histone 3 R3 methyltransferases for drug discovery and HTS applications
Biochem/physiol Actions
H4 (histone 4) is a core histone protein which is mobilized during herpes simplex virus 1 (HSV1) infection. This leads to elevated pools of free H2B and H4 and decreased rate of H2B fast chromatin exchange. Free H4 associates with HSV1 genomes, and this mobilization is also linked with the organization of H2A-H2B and H3-H4 dimers into unstable nucleosomes with HSV-1 genomes. The histone chaperone nuclear autoantigenic sperm protein (NASP) is responsible for maintaining a reservoir of soluble H3-H4 histones, where it regulates the activity of heat shock proteins Hsc70 and Hsp90, which target H3-H4 for degradation by chaperone-mediated autophagy. This reservoir level, in turn, depends upon the level of NASP.
Physical form
Supplied as water solution, 800 μM
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube to recover full content of the tube. Aliquot into single use aliquots. Store remaining product in aliquots at -70°C. Note: Avoid freeze/thaw cycles.
| biological source | human |
| assay | ≥90% (HPLC) |
| form | aqueous solution |
| mol wt | ~2 kDa |
| packaging | pkg of 160 nmol |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | >0.02 mg/mL |
| NCBI accession no. | NM_003548 |
| UniProt accession no. | P62805 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... HIST2H4A(8370) |
