General description
Vesicular glutamate transporter 1 (UniProt: also known as VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7) is encoded by the Slc17a7 (also known as Bnpi, Vglut1) gene (Gene ID: 116638) in rat. VGluT1 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neuronal cells. Glutamate transporters mediate glutamate uptake into synaptic vesicles and are driven by a proton electrochemical gradient generated by the vacuolar H+-ATPase. Two isoforms of VGluT1 have been reported that are produced by alternative splicing. VGluT1 is expressed in the cerebellum, cerebral cortex and hippocampus. Expression of isoform VGluT2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGluT1 and VGluT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGluT2 expression predominates. VGluT1 expression becomes essential for survival at the time of the developmental switch from VGluT2 to VGLUT1. The VGluT1 knockout mice die at the age when a strong upregulation of VGluT1 through newly emerging VGluT1 synapses as well as a re+D4placement of the previously predominant VGLUT2 isoform in cerebellar, hippocampal, and cortical neurons occurs. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wojcik, SM et al. (2004). Proc. Natl. Acad. Sci. USA 101(18): 7158-7163).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of rat Vesicular glutamate transporter 1 (VGlut1).
Application
Anti-VGluT1, clone 4O15 ZooMAb, Cat. No. ZRB2374, is a recombinant Rabbit Monoclonal Antibody that specifically targets Vesicular Glutamate Transporter 1 (aka Slc17a7 or VGlut1) and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected VGluT1 in rat brain and human brain tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected VGluT1 in rat brain and human brain tissue sections.
Biochem/physiol Actions
Clone 4O15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Vesicular glutamate transporter 1. It targets an epitope with in 19 amino acids from the C-terminal cytoplasmic domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4O15, monoclonal,recombinant monoclonal |
| description | 4O15 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 61.66 kDa,observed mol wt ~53 kDa |
| species reactivity | rat, mouse, human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunofluorescence: suitable using 1:100,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100,western blot: suitable using 1:1,000 |
| isotype | IgG |
| UniProt accession no. | Q62634 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | rat ... Slc17a7;Bnpi;Vglut1(116638) |
