General description
HAVCR1 is a trans-membrane glycoprotein that is highly expressed in kidney proximal tubular cells with kidney injury. The KIM-1 ectodomain is cleaved, released into urine, and canbe quantified as a sensitive and specific biomarker for Acute Kidney Injury (AKI) in rodents and humans. In a recent study it was demonstrated that HAVCR1 is released into the circulation where it reports kidney injury in mice, rats, and humans with AKI and chronic kidney disease (CKD). Urinary HAVCR1 and MCP-1 levels, either alone or in combination, were significantly higher than those in AKI negative patients. Importantly, the combination of HAVCR1 and MCP-1 outperformed either biomarker alone.
Immunogen
SVKVGGEAGPSVTLPCHYSGAVTSMCWNRGSCSLFTCQNGIVWTNGTHVTYRKDTRYKLLGDLSRRDVSLTIENTAVSDSGVYCCRVEHRGWFNDMKITVSLEIVPPKVTTTPIVTTVPTVTTVRTSTTVPTTTTVPTTTVPTTMSIPTTTTVLTTMTVSTTTSVPTTTSIPTTTSVPVTTTVSTFVPPMPLPRQNHEPVATSPSSPQPAETHPTTLQGAIRREPTSSPLYSYTTDGNDTVTESSDGLWNNNQTQLFLEHSLLTANTTKGDYKDDDDKGHHHHHHHHGGQ
Biochem/physiol Actions
SILu™Lite HAVCR1 is a recombinant human protein expressed in human 293 cells. It is a protein consisting of 290 amino acids acids (the extracellular domain: Ser21-Gly290, and including C-terminal polyhistidine and FLAG® tags), with a calculated molecular mass of 31 kDa. SILu Lite HAVCR1 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.
Physical form
Supplied as a lyophilized powder containing phosphate buffered saline.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
SILu is a trademark of Sigma-Aldrich Co. LLC
| biological source | human |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| tag | 8-His tagged |
| assay | ≥98% (SDS-PAGE) |
| form | lyophilized powder |
| potency | ≥98% Heavy amino acids incorporation efficiency by MS |
| mol wt | calculated mol wt 31 kDa |
| technique(s) | mass spectrometry (MS): suitable |
| suitability | suitable for mass spectrometry (internal calibrator) |
| UniProt accession no. | Q96D42 |
| storage temp. | −20°C |
| Gene Information | human ... HAVCR1(26762) |
