产品介绍
Product Description
General description
单克隆抗标记 M2-Cy3(小鼠IgG)抗体与花菁染料 Cy3 共价偶联。抗体缀合物结合FLAG融合蛋白,并且将在FLAG融合蛋白的N末端、Met-N末端或C末端以识别FLAG序列。
Application
实现简单的一步式免疫细胞化学检测。 特别适用于小鼠宿主表达FLAG融合蛋白的检测,这种检测使用抗小鼠二抗可能会发生交叉反应。
请访问我们的FLAG®应用页面,了解更多产品详情。
此抗体已成功使用的应用,以及相关的同行评审论文如下。
蛋白免疫分析(Western Blotting)(1篇文章)
Physical form
溶于磷酸盐缓冲盐水,含有 1%BSA 和防腐剂
Preparation Note
用 Tris 缓冲盐水 (TBS) 稀释抗体:0.05 MTris,pH 7.4,含 0.15 M NaCl。
Other Notes
免疫细胞化学的建议浓度为1-10 mg / ml。
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
技术参数
Specifications
| biological source | mouse |
| Quality Level | 200 |
| conjugate | CY3 conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | M2, monoclonal |
| form | buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate) |
| species reactivity | all |
| concentration | ~1 mg/mL |
| technique(s) | direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone |
| isotype | IgG1 |
| immunogen sequence | DYKDDDDK |
| shipped in | dry ice |
| storage temp. | −20°C |

