产品介绍
Product Description
Application
Bisindolylmaleimide X hydrochloride has been used as a protein kinase C (PKC) inhibitor:
- in chemotaxis assays
- to inhibit protein kinase C and to study its effects on the expression of EGR1, NAB2, ZEBRA, and Rta
- in the culture to study its effects on the expression of the kinase-insert domain-containing receptor (KDR)-B1, protein kinase Cθ (PKCθ)-M1a or Abl-HTa and on cell yield in baculovirus (BV)-infected insect cells
Biochem/physiol Actions
Bisindolylmaleimide X hydrochloride/Ro 31-8425, a strong and selective protein kinase C (PKC) inhibitor, can reduce the superoxide burst caused by various agonists in neutrophils. It can suppress the responses induced by cell surface receptors and phorbol esters in T cells. In humans, Ro 31-8425 can prevent neutrophil PKC in vitro with an IC50 of 5nM.
技术参数
Specifications
| biological source | synthetic (organic) |
| Quality Segment | 200 |
| assay | ≥90% |
| form | solid |
| solubility | DMSO: soluble |
| storage temp. | −20°C |
| SMILES string | Cl.Cn1cc(C2=C(C(=O)NC2=O)c3c4CC(CN)CCn4c5ccccc35)c6ccccc16 |
| InChI | 1S/C26H24N4O2.ClH/c1-29-14-18(16-6-2-4-8-19(16)29)23-24(26(32)28-25(23)31)22-17-7-3-5-9-20(17)30-11-10-15(13-27)12-21(22)30;/h2-9,14-15H,10-13,27H2,1H3,(H,28,31,32);1H |
| InChI key | IMBOYWXMTUUYGZ-UHFFFAOYSA-N |
