Biochem/physiol Actions
The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them.
The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
| biological source | simian virus |
| recombinant | expressed in insect cells |
| assay | ≥80% (SDS-PAGE) |
| form | frozen liquid |
| mol wt | ~83.3 kDa |
| packaging | pkg of 5 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | 400 μg/mL |
| color | clear colorless |
| NCBI accession no. | AAG39214.1 |
| UniProt accession no. | P03070 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | simian virus ... SV40gp6(1489531) |
