General description
Class II transactivator protein (CIITA) protein contains an acidic region in its N-terminal with transactivation activity, that binds to proteins in the basal transcription machinery and to cAMP response element-binding protein (CREB)-binding protein (CBP). The protein contains motifs for nuclear translocation, GTP binding motif and C-terminal leucine-rich repeats. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ .
Monoclonal Anti-CIITA (mouse IgG1 isotype) is derived from the hybridoma 7-1H produced by the fusion of mouse myeloma cells (Sp20 cells) and splenocytes from BALB/c mice immunized with purified N-terminal recombinant CIITA, amino acids 1-350. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ.
Monoclonal Anti-CIITA recognizes human1 and mouse † CIITA.
Application
The antibody may be used in immunoblotting (∼130 kDa) and immunocytochemistry.
Biochem/physiol Actions
Class II transactivator protein (CIITA) interacts with specific transcription factors and creates the appropriate transcriptional scaffold required for HLA-D gene expression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7-1H, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~130 kDa |
| species reactivity | mouse, human |
| concentration | ~2 mg/mL |
| technique(s) | immunocytochemistry: suitable,western blot: 2-4 μg/mL using total cell extract of Raji cells |
| isotype | IgG1 |
| UniProt accession no. | P33076 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CIITA(4261) mouse ... Ciita(12265) |
