General description
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human AKT (NP_005154.2).
Physical form
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Preparation Note
Store at -20℃. Avoid freeze / thaw cycles.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | 48, 55 |
| species reactivity | human, mouse, rat |
| concentration | 1.87 mg/ml |
| technique(s) | immunofluorescence: 1:50-1:100,immunohistochemistry: 1:50-1:100,immunoprecipitation (IP): 1:50-1:200,western blot: 1:500-1:2000 |
| UniProt accession no. | P31749 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... AKT1(207) |
