General description
This product is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. It is designed to be used as a supplement to tissue culture medium for the prevention of secreted proteins degradation. The product has been found to be non-toxic after a 48 hours exposure to the following adherent cell lines: A431, CHO, COS, HepG2, and HeLa, and to following non-adherent cell lines: Jurkat and HL-60. This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0006 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P1860.
Features and Benefits
- Does not freeze in storage for quick, convenient use.
- Inhibits serine, cysteine, aspartic proteases and aminopeptidases.
- Tailored for use in tissue culture media.
- Suggested as 100 X solution.
- Reclosable screw-top vials.
- Can be stored in cooler for approx. 1 month or room temperature for a week.
Preparation Note
It is recommended to determine the appropriate dilution for each specific cell line. This testing should begin with a dilution of at least a 200-fold to prevent deleterious effect of the solvent to cell growth.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany
| description | Tissue Culture Media |
| Quality Level | 100 |
| form | ready-to-use solution |
| quality | 200 |
| packaging | pkg of 1 mL (in amber polypropylene vial),pkg of 5 mL (in amber polypropylene vial) |
| solubility | water: soluble |
| web metadata keyword.default | Extracts, Lysate, Cell Extracts, Tissue Extracts, Mammalian Tissue Extract, Bacterial lysate, Electrophoresis, peptidase inhibitor, proteinase inhibitor, Cell culture |
| storage temp. | −20°C |
