General description
Alpha-actinin-1 (UniProt: P12814; also known as Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1) is encoded by the ACTN1 gene (Gene ID: 87) in human. α-Actinin is a homodimeric, non-muscle cytoskeletal protein that serves as F-actin cross-linker protein and anchors actin to a variety of intracellular structures. It is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. Its actin binding region is localized in amino acids 1-247. It contains four spectrin repeats and has two calponin-homology (CH) domains (aa 31-135 and 144-250). α-Actinin is reported to promote activity of the L-type Ca2+ channel Cav1.2. It recruits CaV1.2 to defined surface regions and boost its open probability. Overexpression of α-actinin is frequently observed in human breast cancers. In mammary epithelial cells, higher levels of α-actinin are shown to promote cell migration accompanied by a major reorganization of the actin cytoskeleton and the associated E-cadherin-based adhesions. Mutations in ACTN1 gene are associated with platelet type bleeding disorder (BDPLT15) that causes a reduction in the number of large platelets and mild bleeding tendency. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Turner, M., et al. (2020). EMBO J. 39(5); e102622; Kovac, B., et al. (2018). PLoS One. 13(5):e0196986).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
Purified, full-length native Actinin from human platelets.
Application
Quality Control Testing
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Actinin in A431 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Actinin in HeLa, NIH3T3, and C6 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Actinin in Human liver tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Actinin in HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone AT6/172 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects - Actinin.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | AT6/172, recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 103.06 kDa,observed mol wt ~110 kDa |
| purified by | using protein G |
| species reactivity | human, rat, mouse |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| epitope sequence | Unknown |
| Protein ID accession no. | NP_001123476.1 |
| UniProt accession no. | P12814 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... ACTN1(87) |
