General description
An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. In dogs four subclasses of IgG (IgG1 -IgG4) are present.
Dog IgGs are glycoprotein antibodies that regulate several immunological responses. Canine IgGs can be used as markers for detecting immune-mediated haemolytic anaemia, adverse food reactions and also for assessing the risk of L. infantum transmission in dogs.
Immunogen
IgG isolated from pooled normal dog serum
Application
Anti-Dog IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA), and immunoblotting.
Biochem/physiol Actions
Anti-Dog IgG (whole molecule) antibody is specific for IgG in dogs when tested against normal dog serum and dog IgG by double-diffusion and immunoelectrophoretic assays prior to conjugation.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | alkaline phosphatase conjugate |
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| species reactivity | dog |
| technique(s) | direct ELISA: 1:6,000,dot blot: 1:4,000,western blot (chemiluminescent): 1:4,000 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
