General description
Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase.
Application
Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor.
Biochem/physiol Actions
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity.
Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells.
Other Notes
One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C.
Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 60 mM KCl, 20% (w/v) Glycerol
| biological source | bacterial ( |
| Quality Level | 200 |
| recombinant | expressed in E. coli |
| description | Histidine tagged |
| assay | 90% (SDS-PAGE) |
| form | solution |
| specific activity | ≥500 units/mg protein |
| mol wt | 85 kDa |
| technique(s) | cell based assay: suitable |
| suitability | suitable for molecular biology |
| application(s) | cell analysis |
| shipped in | dry ice |
| storage temp. | −70°C |
