General description
The Human PGF (placenta growth factor) ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PGF in serum, plasma, cell culture supernatants and urine.
The PGF (placental growth factor) gene codes for a proangiogenic factor and is mapped to human chromosome 14q24.3. PGF belongs to the vascular endothelial growth factor (VEGF) family of growth factors. It is predominantly expressed in placenta.
Immunogen
Recombinant Human PLGF
Application
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Placental Growth Factor ELISA Kit has been used to determine PGF in the medium culture waste and cellular suspension.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Reduced levels of placental growth factor (PGF) indicates restricted growth in the fetus. Increased stimulation of PGF is observed in cancers and hind limb ischemia and in wound healing. Hypoxic conditions upregulate PGF expression. Increased expression of PGF gene is observed in cutaneous T-cell lymphoma and it supports in the cell proliferation process.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
| species reactivity | human |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable,capture ELISA: suitable |
| input | sample type urine sample type serum sample type plasma sample type cell culture supernatant(s) |
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 1.372-1000 pg/mL |
| detection method | colorimetric |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... PGF(5228) |
