General description
FAM65B (family with sequence similarity 65, member B), also known as chromosome 6 open reading frame 32 (C6ORF32), is mapped to human chromosome 6p22.3. FAM65B is known to be expressed during embryonic and postnatal development stages in cochlea. The encoded protein is found to concentrate in the plasma membranes of the stereocilia of inner and outer hair cells of the inner ear.
Immunogen
C6orf32 (AAH01232.1, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MLVGSQSFSPGGPNGIIRSQSFAGFSGLQERRSRCNSFIENSSALKKPQAKLKKMHNLGHKNNNPPKEPQPKRVEEVYRALKNGLDEYLEVHQTELDKLTAQLKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEAYCIQRRLQDGASKMKQAFATSPASKAARESLTEINRSFKEYTENMCTIEVELENLLGEFSIKMKGLAGFARLCPGDQYEIFMKYGRQRWKLKGKIEVNGKQSWDGEETVFLPLIVGFISIKVTELKGLATHILVGSVTCETKELFAARPQVVAVDINDLGTIKLNLEITWYPFDVEDMTASSGAGNKAAALQRRMSMYSQGTPETPTFKDHSFFSNLPDDIFENGKAAEEKMPLSLSFSDLPNGDCALTSHSTGSPSNSTNPEITITPAEFNLSSLASQNEGMDDTSSASSRNSLGEGQEPKSHLKEEDPEEPRKPASAPSEACRRQSSGAGAEHLFLENDVAEALLQESEEASELKPVELDTSEGNITKQLVKRLTSAEVPMATDRLLSEGSVGGESEGCRSFLDGSLEDAFNGLLLALEPHKEQYKEFQDLNQEVMNLDDILKK
Biochem/physiol Actions
FAM65B (family with sequence similarity 65, member B) is recognized as a Ras homolog gene family, member A (RHOA) inhibitor and is involved in polarization. FAM65B regulates RhoA activity with the help of forkhead box O transcription factors. It binds to RhoA and blocks the GTP loading to RhoA. FAM65B deficiency in neutrophils leads to an increase in RhoA activity and causes defects in chemotaxis directionality and adhesion to endothelial cells under flow. It is involved in chemokine-stimulated migration and adhesion of Jurkat cells. Phosphorylation stabilizes FAM65B. FAM65B is a component of the inner ear and is involved in the process of hearing. Mutation in the gene is known to be associated with hearing loss in humans. FAM65B is associated with myotube formation and in the regulation of cell adhesion, polarization, and migration. Overexpression of the protein in myogenic and non-myogenic cells results in the formation of cellular protrusions. Hence, FAM65B might be involved in myoblast migration or cytoskeletal protein rearrangements that accompany myogenic differentiation.
Physical form
Solution in phosphate buffered saline, pH 7.4
Legal Information
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| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 2E6-1B10, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| technique(s) | indirect ELISA: suitable,western blot: 1-5 μg/mL |
| isotype | IgG2bκ |
| GenBank accession no. | BC001232 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... FAM65B(9750) |
