General description
CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
Immunogen
Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
Biochem/physiol Actions
The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
Physical form
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | Allophycocyanin conjugate |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | HM47, monoclonal |
| form | buffered aqueous solution |
| species reactivity | ( Chicken), dog, ( Rabbit), ( Guinea pig) |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1κ |
| UniProt accession no. | P11912 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CD79A(973) |
