产品介绍
Product Description
General description
小鼠抗-FLAG M2抗体，可与含有FLAG肽序列结合的亲和纯化单克隆抗体。该抗体可识别FLAG肽序列的N-末端、Met-N-末端、C-末端和融合蛋白的内部位点。
纯化方法 - 亲和标签纯化
Immunogen
FLAG；肽序列DYKDDDDK
Application
用于通过免疫印迹、免疫沉淀（IP)、免疫组化、免疫荧光及免疫细胞化学进行FLAG融合蛋白的高灵敏度及特异性检测。 优化用于哺乳动物、植物和细菌表达系统中FLAG融合蛋白的单条带检测。
蛋白质免疫印迹和EIA
更多产品信息请访问我们的FLAG®应用页面。
Biochem/physiol Actions
结合位点：N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Physical form
在磷酸盐缓冲液中含有50%甘油。
Preparation Note
在立即使用前用3%脱脂牛奶稀释于Tris缓冲盐溶液（TBS），pH 8.0
Other Notes
2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
技术参数
Specifications
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | M2, monoclonal |
| form | buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) |
| purified by | affinity chromatography (affinity tag purification) |
| species reactivity | all |
| concentration | 1.0-1.2 mg/mL |
| isotype | IgG1 |
| immunogen sequence | DYKDDDDK |
| shipped in | wet ice |
| storage temp. | −20°C |
