General description
RIG-1 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKε through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.
Immunogen
RIG-1 antibody was raised against human GST-tagged RIG-1 protein.
Application
RIG-1 antibody can be used for the detection of RIG-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human and rat samples. All other applications and species not yet tested.
Physical form
Supplied in PBS containing 0.02% sodium azide.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody (DEAE) |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| species reactivity | rat, mouse, human |
| concentration | 1 mg/mL |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | O95786 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... DDX58(23586) |
