General description
RNase Inhibitor inactivates a wide spectrum of RNases, including:RNase ARNase BRNase T2Therefore, RNase Inhibitor prevents RNase degradation in any application where RNases could cause problems:As used in RNase protection assays.Protect mRNA during cDNA synthesis reactions or RT-PCR in conventional thermal cyclers and qPCR systems.
Application
RNase Protection Assays: RNase Inhibitor is employed in RNase protection assays to safeguard RNA from degradation during the experimental procedure. This application is crucial for accurately measuring RNA levels and studying RNA-protein interactions.
cDNA Synthesis: The enzyme is used to protect mRNA during cDNA synthesis reactions, ensuring that the RNA template remains intact and available for conversion to complementary DNA (cDNA). This is essential for downstream applications that rely on high-quality cDNA.
RT-PCR and qPCR: RNase Inhibitor plays a critical role in reverse transcription reactions and quantitative PCR (qPCR) by preventing RNase degradation of RNA templates. This protection is vital for achieving reliable and reproducible amplification of RNA.
RNA Isolation Protocols: The enzyme can be included in RNA isolation protocols to protect RNA from endogenous RNases during extraction processes, ensuring the recovery of high-quality RNA.
Functional RNA Studies: RNase Inhibitor is beneficial in experiments involving functional RNA, such as RNA interference (RNAi) and RNA-based therapeutics, where RNA integrity is paramount for successful outcomes.
Features and Benefits
Scalable mRNA Synthesis: Designed for scalable mRNA production, suitable for both small-scale laboratory research and large-scale applications in biotechnology and pharmaceuticals.
Broad Spectrum Activity: RNase Inhibitor effectively inactivates a wide range of RNases, providing comprehensive protection for RNA in various experimental settings.
Enhanced RNA Integrity: By preventing RNase degradation, the enzyme ensures the integrity of RNA samples, which is critical for accurate experimental results.
Versatile Use: RNase Inhibitor is compatible with a variety of applications, including cDNA synthesis, RT-PCR, and RNA isolation, making it a valuable tool in molecular biology.
Improved Reproducibility: The use of RNase Inhibitor contributes to the reproducibility of experiments by minimizing variability caused by RNA degradation.
| form | (In Glycerol) |
| packaging | vial of 20 kU |
| manufacturer/tradename | Roche 10428007001 |
| storage temp. | −20°C |
